Increasing the Performance of Hybridisation- Based Target Enrichment For NGS.

An enhanced method for enriching genomic DNA for sequencing studies.

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Background.

Hybridisation Target Enrichment (HTE) is the most commonly used genomic DNA enrichment strategy in NGS studies, but the method has not significantly advanced in recent years. Researchers have now developed products and strategies that significantly improve the performance of HTE.

  • Novel multifunctional blockers reduce non-specific sequence recovery almost 4x as effectively as commonly used DNA equivalents ( Figure 1 ).
  • Innovative approach to target recovery requires 10x fewer probes than other products e.g. >180kb of genomic bases evenly captured with ~2500 probes ( Figure 2 ).
  • High efficiency probe genera on strategy dramatically lowers reagent costs.

Whole genome sequencing is not yet cost effective for most applications, so hybridisation targeted enrichment (HTE) remains popular. Existing HTE methods produce: Limited enrichment power, such that they are poorly useful unless targeting >1Mb Uneven recovery, such that many variants are missed or unreliably detected.

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